Digital_Repository/Misc/Mass downloads/UTas/2129.html at 306ce8fa1f6a963a67d8f587e4c2fecc30412ae0

nigel.stanger / Digital_Repository
Find file
Newer
Older
Digital_Repository / Misc / Mass downloads / UTas / 2129.html
Nigel Stanger on 7 May 2013 35 KB - Imported Misc directory.
Raw Blame History
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN"
"http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html>
  <head>
    <title>UTas ePrints - Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean</title>
    <script type="text/javascript" src="http://eprints.utas.edu.au/javascript/auto.js"><!-- padder --></script>
    <style type="text/css" media="screen">@import url(http://eprints.utas.edu.au/style/auto.css);</style>
    <style type="text/css" media="print">@import url(http://eprints.utas.edu.au/style/print.css);</style>
    <link rel="icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
    <link rel="shortcut icon" href="/images/eprints/favicon.ico" type="image/x-icon" />
    <link rel="Top" href="http://eprints.utas.edu.au/" />
    <link rel="Search" href="http://eprints.utas.edu.au/cgi/search" />
    <meta content="Abell, G.C.J." name="eprints.creators_name" />
<meta content="Bowman, John P." name="eprints.creators_name" />
<meta content="" name="eprints.creators_id" />
<meta content="john.bowman@utas.edu.au" name="eprints.creators_id" />
<meta content="article" name="eprints.type" />
<meta content="2007-10-14 23:29:43" name="eprints.datestamp" />
<meta content="2008-01-08 15:30:00" name="eprints.lastmod" />
<meta content="show" name="eprints.metadata_visibility" />
<meta content="Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean" name="eprints.title" />
<meta content="pub" name="eprints.ispublished" />
<meta content="270307" name="eprints.subjects" />
<meta content="restricted" name="eprints.full_text_status" />
<meta content="Southern Ocean; DGGE; Flavobacteria; Cytophaga–Flavobacterium–Bacteroides group; Biogeography; Psychrophilic bacteria" name="eprints.keywords" />
<meta content="The definitive version is available at http://www.sciencedirect.com/
" name="eprints.note" />
<meta content="The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade (&quot;DE cluster 2&quot;) that is distributed throughout the Southern Ocean.

" name="eprints.abstract" />
<meta content="2005" name="eprints.date" />
<meta content="FEMS Microbiology Ecology" name="eprints.publication" />
<meta content="51" name="eprints.volume" />
<meta content="265-277" name="eprints.pagerange" />
<meta content="10.1016/j.femsec.2004.09.001" name="eprints.id_number" />
<meta content="TRUE" name="eprints.refereed" />
<meta content="http://dx.doi.org/10.1016/j.femsec.2004.09.001" name="eprints.official_url" />
<meta content="[1]Fenchel, T. (1988) Marine plankton food-chains. Annu. Rev. Ecol. Syst. 18, 19–38.

[2]Cole, J.J, Findlay, S., Pace, M.L. (1988) Bacterial production in fresh and saltwater ecosystems – a cross-system overview. Mar. Ecol. Prog. Ser. 43, 1–10.
ISI
[3]Azam, F. (1998) Microbial control of oceanic carbon flux: The plot thickens. Science 280, 694–696.
CrossRef, ISI
[4]Bidle, K.D., Brzezinski, M.A., Long, R.A., Jones, J.L., Azam, F. (2003) Diminished efficiency in the oceanic silica pump caused by bacteria-mediated silica dissolution. Limnol. Oceanogr. 48, 1855–1868.
ISI
[5]Brown, G.E., Parks, G.A. (2001) Sorption of trace elements on mineral surfaces: Modern perspectives from spectroscopic studies, and comments on sorption in the marine environment. Int. Geol. Rev. 43, 963–1073.
ISI
[6]Kirchman, D.L. (2002) The ecology of Cytophaga–Flavobacteria in aquatic environments. FEMS Microbiol. Ecol. 39, 91–100.
Synergy, ISI, Chemport
[7]Kirchman, D.L., Yu, L.Y., Cottrell, M.T. (2003) Diversity and abundance of uncultured Cytophaga-like bacteria in the Delaware Estuary. Appl. Environ. Microbiol. 69, 6587–6596.
CrossRef, Medline, ISI, Chemport
[8]O'sullivan, L.A., Fuller, K.E., Thomas, E.M., Turley, C.M., Fry, J.C., Weightman, A.J. (2004) Distribution and culturability of the uncultivated 'AGG58 cluster' of the Bacteroidetes phylum in aquatic environments. FEMS Microbiol. Ecol. 47, 359–370.
Synergy, ISI
[9]DeLong, E.F., Franks, D.G., Alldredge, A.L. (1993) Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924–934.
ISI
[10]Bowman, J.P., Mancuso Nichols, C.A., Gibson, J.A.E. (2003) Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov., Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int. J. Syst. Evol. Microbiol. 53, 1343–1355.
Medline, ISI
[11]Weller, R., Glöckner, F.O., Amann, R. (2000) 16S rRNA-targeted oligonucleotide probes for the in situ detection of members of the phylum Cytophaga–Flavobacterium–Bacteroides. Syst. Appl. Microbiol. 23, 107–114.
Medline, ISI, Chemport
[12]Ploug, H., Grossart, H.P., Azam, F., Jorgensen, B.B. (1999) Photosynthesis, respiration, and carbon turnover in sinking marine snow from surface waters of Southern California Bight: implications for the carbon cycle in the ocean. Mar. Ecol. Prog. Ser. 179, 1–11.
ISI
[13]Brown, M.V., Bowman, J.P. (2001) A molecular phylogenetic survey of sea-ice microbial communities (SIMCO). FEMS Microbiol. Ecol. 35, 267–275.
Synergy, Medline, ISI, Chemport
[14]Brinkmeyer, R., Knittel, K., Jurgens, J., Weyland, H., Amann, R., Helmke, E. (2003) Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice. Appl. Environ. Microbiol. 69, 6610–6619.
CrossRef, Medline, ISI, Chemport
[15]Llobet-Brossa, E., Rossello-Mora, R., Amann, R. (1998) Microbial community composition of Wadden Sea sediments as revealed by fluorescence in situ hybridization. Appl. Environ. Microbiol. 64, 2691–2696.
Medline, ISI
[16]Bowman, J.P., McCuaig, R.D. (2003) Diversity and biogeography of prokaryotes dwelling in Antarctic continental shelf sediment. Appl. Environ. Microbiol. 69, 2463–2484.
CrossRef, Medline, ISI
[17]Webster, N.S., Negri, A.P., Munro, M.M.H.G., Battershill, C.N. (2004) Diverse microbial communities inhabit Antarctic sponges. Environ. Microbiol. 6, 288–300.
Synergy, Medline, ISI
[18]Cottrell, M.T., Kirchman, D.L. (2000) Natural assemblages of marine proteobacteria and members of the Cytophaga–Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66, 1692–1697.
CrossRef, Medline, ISI, Chemport
[19]Mittelbach, G.G., Steiner, C.F., Steiner, S.M., Gross, K.L., Reynolds, H.L., Waide, R.B., Willig, M.R., Dodson, S.I., Gough, L. (2001) What is the observed relationship between species richness and productivity?. Ecology 82, 2381–2396.
CrossRef, ISI
[20]Horner-Devine, C.M., Leibold, M.A., Smith, V.H., Bohannan, B.J.M. (2003) Bacterial diversity patterns along a gradient of primary productivity. Ecol. Lett. 6, 613–622.
Synergy, ISI
[21]Kopczynska, E.E., Dehairs, F., Elskens, M., Wright, S. (2001) Phytoplankton and microzooplankton variability between the Subtropical and Polar Fronts south of Australia: Thriving under regenerative and new production in late summer. J. Geophys. Res.-Oceans 106, 31597–31609.
CrossRef, ISI
[22]Glöckner, F.O., Fuchs, B.M., Amann, R. (1999) Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 65, 3721–3726.
Medline, ISI, Chemport
[23]Lebaron, P., Servais, P., Troussellier, M., Courties, C., Vives-Rego, J., Muyzer, G., Bernard, L., Guindulain, T., Schafer, H., Stackebrandt, E. (1999) Changes in bacterial community structure in seawater mesocosms differing in their nutrient status. Aquat. Microb. Ecol. 19, 255–267.
CrossRef, ISI
[24]Fuhrman, J.A., Comeau, D.E., Hagstrom, A., Chan, A.M. (1988) Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl. Environ. Microbiol. 54, 1426–1429.
Medline, ISI, Chemport
[25]Lane, D.J. (1991) 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt, E., Goodfellow, M., Eds.), pp.115–175 John Wiley &amp; Sons, New York.

[26]Ferris, M.J., Muyzer, G., Ward, D.M. (1996) Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl. Environ. Microbiol. 62, 340–346.
Medline, ISI, Chemport
[27]Powell, S.M., Bowman, J.P., Snape, I., Stark, J.S. (2003) Microbial community variation in pristine and polluted nearshore Antarctic sediments. FEMS Microbiol. Ecol. 45, 135–145.
Synergy, ISI, Chemport
[28]Clarke, K.R. and Warwick, R.M. (2001) Change in marine communities: an approach to statistical analysis and interpretation, 2nd ed. Primer-E Ltd., Plymouth, UK. (http://www.pml.ac.uk/primer/)

[29]Schiffman, S.S., Reynolds, M.L. (1981) Introduction to Multidimensional Scaling. Theory, Methods and Applications. Academic Press, London, UK.

[30]Hall, T.A. (1999) A user-friendly biological sequence alignment editor for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41, pp. 95–98

[31]Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994) Clustal-W- improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673–4680.
Medline, ISI
[32]Felsenstein, J. (1993) PHYLIP, phylogenetic inference package. http://evolution.genetics.washington.edu/phylip.html

[33]Ouverney, C.C., Fuhrman, J.A. (1997) Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol. Appl. Environ. Microbiol. 63, 2735–2740.
Medline, ISI
[34]Bano, N., Hollibaugh, J.T. (2002) Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean. Appl. Environ. Microbiol. 68, 505–518.
CrossRef, Medline, ISI, Chemport
[35]Simon, M., Glöckner, F.O., Amann, R. (1999) Different community structure and temperature optima of heterotrophic picoplankton in various regions of the Southern Ocean. Aquat. Microb. Ecol. 18, 275–284.
CrossRef, ISI
[36]de Baar, H.J.W., Buma, A.G.J., Nolting, R.F., Cadee, G.C., Jacques, G., Treguer, P.J. (1990) On iron limitation of the Southern-Ocean – experimental-observations in the Weddell and Scotia seas. Mar. Ecol. Prog. Ser 65, 105–122.

[37]Timmermans, K.R., van Leeuwe, M.A., de Jong, J.T.M., McKay, R.M.L., Nolting, R.F., Witte, H.J., van Ooyen, J., Swagerman, M.J.W., Kloosterhuis, H., de Baar, H.J.W. (1998) Iron stress in the Pacific region of the Southern Ocean: evidence from enrichment bioassays. Mar. Ecol. Prog. Ser. 166, 27–41.
ISI
[38]Vincent, W.F. (1988) Microbial Ecosystems of Antarctica. Cambridge University Press, Cambridge, UK.

[39]Hence, I., Zimmermann, R., Beckman, A., Batman, U.V. (2003) Regional ecosystem dynamics in the ACC: simulations with a three-dimensional ocean-plankton model. J. Mar. Syst. 42, 31–51.

[40]Murray, A.E., Wu, K.Y., Moyer, C.L., Karl, D.M., de Long, E.F. (1999) Evidence for circumpolar distribution of planktonic Archaea in the Southern Ocean. Aquat. Microb. Ecol. 18, 263–273.
ISI
[41]Selje, N., Simon, M., Brinkhoff, T. (2004) A newly discovered Roseobacter cluster in temperate and polar oceans. Nature 427, 445–448.
CrossRef, Medline, ISI, Chemport
[42]Long, R.A., Azam, F. (2001) Microscale patchiness of bacterioplankton assemblage richness in seawater. Aquat. Microb. Ecol. 26, 103–113.
CrossRef, ISI
[43]Bowman, J.P., McCammon, S.A., Brown, M.V., Nichols, D.S., McMeekin, T.A. (1997) Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl. Environ. Microbiol. 63, 3068–3078.
Medline, ISI, Chemport
[44]Bowman, J.P., McCammon, S.A., Brown, J.L., Nichols, P.D., McMeekin, T.A. (1997) Psychroserpens burtonensis gen. nov., sp. nov. and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int. J. Syst. Bacteriol. 47, 670–677.
Medline
[45]Gosink, J.J., Woese, C.R., Staley, J.T. (1998) Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov., P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of &quot;Flectobacillus glomeratus&quot; as Polaribacter glomeratus comb. nov. Int. J. Syst. Bacteriol. 48, 223–235.

[46]Zubkov, M.V., Fuchs, B.M., Archer, S.D., Kiene, R.P., Amann, R., Burkill, P.H. (2002) A population of the alpha-proteobacteria dominates the bacterioplankton and dimethylsulphoniopropionate uptake after an algal bloom in the North Sea. Deep-sea Res. II, Top. Stud. Oceanogr. 49, 3017–3038.
ISI
[47]Beja, O., Suzuki, M.T., Koonin, E.V., Aravind, L., Hadd, A., Nguyen, L.P., Villacorta, R., Amjadi, M., Garrigues, C., Jovanovich, S.B., Feldman, R.A., DeLong, E. (2000) Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage. Environ. Microbiol. 2, 516–529.
Synergy, Medline, ISI, Chemport
[48]Casamayor, E.O., Schafer, H., Baneras, L., Pedros-Alio, C., Muyzer, G. (2000) Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: Comparison by microscopy and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 66, 499–508.
CrossRef, Medline, ISI, Chemport
[49]Deacon, G.E.R. (1984) The Antarctic Circumpolar Ocean. Cambridge University Press, Cambridge.




" name="eprints.referencetext" />
<meta content="Abell, G.C.J. and Bowman, John P. (2005) Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean. FEMS Microbiology Ecology, 51 . pp. 265-277." name="eprints.citation" />
<meta content="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf" name="eprints.document_url" />
<link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" />
<meta content="Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean" name="DC.title" />
<meta content="Abell, G.C.J." name="DC.creator" />
<meta content="Bowman, John P." name="DC.creator" />
<meta content="270307 Microbial Ecology" name="DC.subject" />
<meta content="The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade (&quot;DE cluster 2&quot;) that is distributed throughout the Southern Ocean.

" name="DC.description" />
<meta content="2005" name="DC.date" />
<meta content="Article" name="DC.type" />
<meta content="PeerReviewed" name="DC.type" />
<meta content="application/pdf" name="DC.format" />
<meta content="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf" name="DC.identifier" />
<meta content="http://dx.doi.org/10.1016/j.femsec.2004.09.001" name="DC.relation" />
<meta content="Abell, G.C.J. and Bowman, John P. (2005) Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean. FEMS Microbiology Ecology, 51 . pp. 265-277." name="DC.identifier" />
<meta content="http://eprints.utas.edu.au/2129/" name="DC.relation" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/BibTeX/epprod-eprint-2129.bib" title="BibTeX" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/ContextObject/epprod-eprint-2129.xml" title="OpenURL ContextObject" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/ContextObject::Dissertation/epprod-eprint-2129.xml" title="OpenURL Dissertation" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/ContextObject::Journal/epprod-eprint-2129.xml" title="OpenURL Journal" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/DC/epprod-eprint-2129.txt" title="Dublin Core" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/DIDL/epprod-eprint-2129.xml" title="DIDL" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/EndNote/epprod-eprint-2129.enw" title="EndNote" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/HTML/epprod-eprint-2129.html" title="HTML Citation" type="text/html; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/METS/epprod-eprint-2129.xml" title="METS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/MODS/epprod-eprint-2129.xml" title="MODS" type="text/xml" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/RIS/epprod-eprint-2129.ris" title="Reference Manager" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/Refer/epprod-eprint-2129.refer" title="Refer" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/Simple/epprod-eprint-2129text" title="Simple Metadata" type="text/plain" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/Text/epprod-eprint-2129.txt" title="ASCII Citation" type="text/plain; charset=utf-8" />
<link rel="alternate" href="http://eprints.utas.edu.au/cgi/export/2129/XML/epprod-eprint-2129.xml" title="EP3 XML" type="text/xml" />

  </head>
  <body bgcolor="#ffffff" text="#000000" onLoad="loadRoutine(); MM_preloadImages('images/eprints/ePrints_banner_r5_c5_f2.gif','images/eprints/ePrints_banner_r5_c7_f2.gif','images/eprints/ePrints_banner_r5_c8_f2.gif','images/eprints/ePrints_banner_r5_c9_f2.gif','images/eprints/ePrints_banner_r5_c10_f2.gif','images/eprints/ePrints_banner_r5_c11_f2.gif','images/eprints/ePrints_banner_r6_c4_f2.gif')">
    
    <div class="ep_noprint"><noscript><style type="text/css">@import url(http://eprints.utas.edu.au/style/nojs.css);</style></noscript></div>




<table width="795" border="0" cellspacing="0" cellpadding="0">
  <tr>
    <td><script language="JavaScript1.2">mmLoadMenus();</script>
      <table border="0" cellpadding="0" cellspacing="0" width="795">
        <!-- fwtable fwsrc="eprints_banner_final2.png" fwbase="ePrints_banner.gif" fwstyle="Dreamweaver" fwdocid = "1249563342" fwnested="0" -->
        <tr>
          <td><img src="/images/eprints/spacer.gif" width="32" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="104" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="44" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="105" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="41" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="16" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="68" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="82" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="69" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="98" height="1" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
        </tr>
        <tr>
          <td colspan="12"><img name="ePrints_banner_r1_c1" src="/images/eprints/ePrints_banner_r1_c1.gif" width="795" height="10" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="10" border="0" alt="" /></td>
        </tr>
        <tr>
          <td rowspan="6"><img name="ePrints_banner_r2_c1" src="/images/eprints/ePrints_banner_r2_c1.gif" width="32" height="118" border="0" alt="" /></td>
          <td rowspan="5"><a href="http://www.utas.edu.au/"><img name="ePrints_banner_r2_c2" src="/images/eprints/ePrints_banner_r2_c2.gif" width="104" height="103" border="0" alt="" /></a></td>
          <td colspan="10"><img name="ePrints_banner_r2_c3" src="/images/eprints/ePrints_banner_r2_c3.gif" width="659" height="41" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="41" border="0" alt="" /></td>
        </tr>
        <tr>
          <td colspan="3"><a href="http://eprints.utas.edu.au/"><img name="ePrints_banner_r3_c3" src="/images/eprints/ePrints_banner_r3_c3.gif" width="190" height="31" border="0" alt="" /></a></td>
          <td rowspan="2" colspan="7"><img name="ePrints_banner_r3_c6" src="/images/eprints/ePrints_banner_r3_c6.gif" width="469" height="37" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="31" border="0" alt="" /></td>
        </tr>
        <tr>
          <td colspan="3"><img name="ePrints_banner_r4_c3" src="/images/eprints/ePrints_banner_r4_c3.gif" width="190" height="6" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="6" border="0" alt="" /></td>
        </tr>
        <tr>
          <td colspan="2"><img name="ePrints_banner_r5_c3" src="/images/eprints/ePrints_banner_r5_c3.gif" width="149" height="1" border="0" alt="" /></td>
          <td rowspan="2" colspan="2"><a href="/information.html" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821132634_0,0,25,null,'ePrints_banner_r5_c5');MM_swapImage('ePrints_banner_r5_c5','','/images/eprints/ePrints_banner_r5_c5_f2.gif',1);"><img name="ePrints_banner_r5_c5" src="/images/eprints/ePrints_banner_r5_c5.gif" width="57" height="25" border="0" alt="About" /></a></td>
          <td rowspan="2"><a href="/view/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133021_1,0,25,null,'ePrints_banner_r5_c7');MM_swapImage('ePrints_banner_r5_c7','','/images/eprints/ePrints_banner_r5_c7_f2.gif',1);"><img name="ePrints_banner_r5_c7" src="/images/eprints/ePrints_banner_r5_c7.gif" width="68" height="25" border="0" alt="Browse" /></a></td>
          <td rowspan="2"><a href="/perl/search/simple" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133201_2,0,25,null,'ePrints_banner_r5_c8');MM_swapImage('ePrints_banner_r5_c8','','/images/eprints/ePrints_banner_r5_c8_f2.gif',1);"><img name="ePrints_banner_r5_c8" src="/images/eprints/ePrints_banner_r5_c8.gif" width="68" height="25" border="0" alt="Search" /></a></td>
          <td rowspan="2"><a href="/perl/register" onMouseOut="MM_swapImgRestore();MM_startTimeout();" onMouseOver="MM_showMenu(window.mm_menu_1018171924_3,0,25,null,'ePrints_banner_r5_c9');MM_swapImage('ePrints_banner_r5_c9','','/images/eprints/ePrints_banner_r5_c9_f2.gif',1);"><img name="ePrints_banner_r5_c9" src="/images/eprints/ePrints_banner_r5_c9.gif" width="68" height="25" border="0" alt="register" /></a></td>
          <td rowspan="2"><a href="/perl/users/home" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133422_4,0,25,null,'ePrints_banner_r5_c10');MM_swapImage('ePrints_banner_r5_c10','','/images/eprints/ePrints_banner_r5_c10_f2.gif',1);"><img name="ePrints_banner_r5_c10" src="/images/eprints/ePrints_banner_r5_c10.gif" width="82" height="25" border="0" alt="user area" /></a></td>
          <td rowspan="2"><a href="/help/" onMouseOut="MM_swapImgRestore();MM_startTimeout()" onMouseOver="MM_showMenu(window.mm_menu_0821133514_5,0,25,null,'ePrints_banner_r5_c11');MM_swapImage('ePrints_banner_r5_c11','','/images/eprints/ePrints_banner_r5_c11_f2.gif',1);"><img name="ePrints_banner_r5_c11" src="/images/eprints/ePrints_banner_r5_c11.gif" width="69" height="25" border="0" alt="Help" /></a></td>
          <td rowspan="3" colspan="4"><img name="ePrints_banner_r5_c12" src="/images/eprints/ePrints_banner_r5_c12.gif" width="98" height="40" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="1" border="0" alt="" /></td>
        </tr>
        <tr>
          <td rowspan="2"><img name="ePrints_banner_r6_c3" src="/images/eprints/ePrints_banner_r6_c3.gif" width="44" height="39" border="0" alt="ePrints home" /></td>
          <td><a href="/" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('ePrints_banner_r6_c4','','/images/eprints/ePrints_banner_r6_c4_f2.gif',1);"><img name="ePrints_banner_r6_c4" src="/images/eprints/ePrints_banner_r6_c4.gif" width="105" height="24" border="0" alt="ePrints home" /></a></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="24" border="0" alt="" /></td>
        </tr>
        <tr>
          <td><img name="ePrints_banner_r7_c2" src="/images/eprints/ePrints_banner_r7_c2.gif" width="104" height="15" border="0" alt="" /></td>
          <td colspan="8"><img name="ePrints_banner_r7_c4" src="/images/eprints/ePrints_banner_r7_c4.gif" width="517" height="15" border="0" alt="" /></td>
          <td><img src="/images/eprints/spacer.gif" width="1" height="15" border="0" alt="" /></td>
        </tr>
      </table></td>
  </tr>
    <tr><td><table width="100%" style="font-size: 90%; border: solid 1px #ccc; padding: 3px"><tr>
      <td align="left"><a href="http://eprints.utas.edu.au/cgi/users/home">Login</a> | <a href="http://eprints.utas.edu.au/cgi/register">Create Account</a></td>
      <td align="right" style="white-space: nowrap">
        <form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/search" style="display:inline">
          <input class="ep_tm_searchbarbox" size="20" type="text" name="q" />
          <input class="ep_tm_searchbarbutton" value="Search" type="submit" name="_action_search" />
          <input type="hidden" name="_order" value="bytitle" />
          <input type="hidden" name="basic_srchtype" value="ALL" />
          <input type="hidden" name="_satisfyall" value="ALL" />
        </form>
      </td>
    </tr></table></td></tr>
  <tr>
    <td class="toplinks"><!-- InstanceBeginEditable name="content" -->


<div align="center">
  
  <table width="720" class="ep_tm_main"><tr><td align="left">
    <h1 class="ep_tm_pagetitle">Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Abell, G.C.J.</span> and <span class="person_name">Bowman, John P.</span> (2005) <xhtml:em>Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean.</xhtml:em> FEMS Microbiology Ecology, 51 . pp. 265-277.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2129/1/Abell_Bowman_2005a.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />634Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="2667" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.1016/j.femsec.2004.09.001">http://dx.doi.org/10.1016/j.femsec.2004.09.001</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade ("DE cluster 2") that is distributed throughout the Southern Ocean.&#13;
&#13;
</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at http://www.sciencedirect.com/&#13;
</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Southern Ocean; DGGE; Flavobacteria; Cytophaga–Flavobacterium–Bacteroides group; Biogeography; Psychrophilic bacteria</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270307.html">270000 Biological Sciences &gt; 270300 Microbiology &gt; 270307 Microbial Ecology</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2129</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc. Prof. John P. Bowman</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">15 Oct 2007 10:29</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2129;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2129">item control page</a></p>
  </td></tr></table>
</div>



    <!-- InstanceEndEditable --></td>
  </tr>
  <tr>
    <td><!-- #BeginLibraryItem "/Library/footer_eprints.lbi" -->
    <table width="795" border="0" align="left" cellpadding="0" class="footer">
  <tr valign="top">
<td colspan="2"><div align="center"><a href="http://www.utas.edu.au">UTAS home</a> | <a href="http://www.utas.edu.au/library/">Library home</a> | <a href="/">ePrints home</a> | <a href="/contact.html">contact</a> | <a href="/information.html">about</a> | <a href="/view/">browse</a> | <a href="/perl/search/simple">search</a> | <a href="/perl/register">register</a> | <a href="/perl/users/home">user area</a> | <a href="/help/">help</a></div><br /></td>
</tr>
<tr><td colspan="2"><p><img src="/images/eprints/footerline.gif" width="100%" height="4" /></p></td></tr>
      <tr valign="top">
        <td width="68%" class="footer">Authorised by the University Librarian<br />
© University of Tasmania ABN 30 764 374 782<br />
      <a href="http://www.utas.edu.au/cricos/">CRICOS Provider Code 00586B</a> | <a href="http://www.utas.edu.au/copyright/copyright_disclaimers.html">Copyright &amp; Disclaimers</a> | <a href="http://www.utas.edu.au/accessibility/index.html">Accessibility</a> | <a href="http://eprints.utas.edu.au/feedback/">Site Feedback</a>  </td>
        <td width="32%"><div align="right">
            <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><img src="http://www.utas.edu.au/shared/logos/unioftasstrip.gif" alt="University of Tasmania Home Page" width="260" height="16" border="0" align="right" /></a></p>
            <p align="right" class="NoPrint"><a href="http://www.utas.edu.au/"><br />
            </a></p>
        </div></td>
      </tr>
      <tr valign="top">
        <td><p>  </p></td>
        <td><div align="right"><span class="NoPrint"><a href="http://www.eprints.org/software/"><img src="/images/eprintslogo.gif" alt="ePrints logo" width="77" height="29" border="0" align="bottom" /></a></span></div></td>
      </tr>
    </table>
    <!-- #EndLibraryItem -->
    <div align="center"></div></td>
  </tr>
</table>

  </body>
</html>